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RaPID Assay PAHs

Home » RaPID Assay PAHs

For detection of Polynuclear Aromatic Hydrocarbons (PAHs) (as phenanthrene) in soil

The RaPID Assay PAH (polyaromatic hydrocarbons) Test Kit can be used as a quantitative, semi-quantitative or qualitative enzyme immunoassay (EIA) for the analysis of PAH in water (groundwater, surface water, well water). For soil application please refer to specific procedure. For applications in other matrices please contact our Technical Service department or refer to the soil application procedure provided. The RaPID Assay PAH Test Kit allows reliable and rapid screening for PAH (measured and reported as phenanthrene) and related compounds, with quantitation between 2.66 and 66.5 (as phenanthrene). The minimum detection level of the kit is 0.93 (as phenanthrene) in water.

Downloads

• Spec sheet (115kb) download
• RaPID Assay PAH User Guide (350kb) download

Product Description

Test Principles
The PAH RaPID Assay kit applies the principles of enzyme linked immunosorbent assay (ELISA) to the determination of PAH and related compounds. The sample to be tested is added, along with an enzyme conjugate, to a disposable test tube, followed by paramagnetic particles with antibodies specific to PAH attached. Both the PAH (which may be in the sample) and the enzyme labeled PAH (the enzyme conjugate) compete for antibody binding sites on the magnetic particles. At the end of an incubation period, a magnetic field is applied to hold the paramagnetic particles (with PAH and labeled PAH analog bound to the antibodies on the particles, in proportion to their original concentration) in the tube and allow the unbound reagents to be decanted. After decanting, the particles are washed with Washing Solution.

The presence of PAH is detected by adding the enzyme substrate (hydrogen peroxide) and the chromogen (3,3',5,5' tetramethylbenzidine). The enzyme labeled PAH analog bound to the PAH antibody catalyzes the conversion of the substrate/chromogen mixture to a colored product. After an incubation period, the reaction is stopped and stabilized by the addition of acid. Since the labeled PAH (conjugate) was in competition with the unlabeled PAH (sample) for the antibody sites, the color developed is inversely proportional to the concentration of PAH in the sample.

The Total PAHs ( sum of 16 PAH compunds) of the indicated contaminant types in soil samples are expressed, at each of the three kit calibrator

(standards) levels, in units comparable to results from GC method 8270 or HPLC method 8310.

Assay Range
- Soil: 0.2 to 5.0 ppmas Phenanthrene
- Water: 0.93 to 66.5 ppb as Phenanthrene
- Range can be extended with additional dilutions.
- Site specific calibration required to meet total PAH analysis.

The PAHs RaPID Assay immunoassay test does not differentiate between phenanthrene and other polyaromatic hydrocarbons.

Required Test Materials SDI Part #
A00156 PAHs Test Kit: 30 tube
A00157 PAHs Test Kit 100 tube
A00160EA SDI Sample Extraction Kit
A00160EB (for soil samples only)
A00236 10X Range Extension Kit
A00237 100X Range Extension Kit

Required Test Equipment
6050100 RaPID Assay Accessory Kit which contains:
A00003 RPA-I RaPID Analyzer
A00004 Magnetic Separation Rack
A00008 Repeator Pipet
A00176 Adjustable Volume
A00014 Pipet Vortex Mixer
A00131 Portable Balance
A00015 Digital Timer
A00009 Repeator Pipet Tips
A00013 Adjustable Pipet Tips